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Background:The aim of the present study was to evaluate the antioxidant effect and the mechanism of total extract and two isolated compounds derived from Coreopsis tinctoria Nutt. on human hepatocyte LO2 injured by hydrogen peroxide in vitro.
Methods: The chemical compounds of Coreopsis tinctoria Nutt. were isolated and identified. The cell model with oxidative damage was set up by adding H2O2 in normal liver cell LO2. LO2 cells were pre-incubated with Coreopsis tinctoria Nutt compounds to test cell viability, nitric oxide release, caspase activity and apoptosis. The protective effect against oxidative damage were measured and the mechanism was analyzed by DPPH radical scavenging activity, ABTS+• radical-scavenging assay and reducing power (Fe3+) assay
Results: The study showed that incubation with Coreopsis tinctoria Nutt caused significant increase in the viability of LO2 cell, decrease of nitric oxide release, caspase activity and cell apoptosis in LO2 cell injured by hydrogen peroxide. The Coreopsis tinctoria Nutt and its two compounds showed antioxidant abilities.
Conclusions: Coreopsis tinctoria Nutt protect normal human liver cells against oxidative induced DNA damage and apoptosis by reducing nitric oxide release. One mechanism of protective effect may be radical-scavenging which is via donating hydrogen atom (H·), donating electron (e).
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